mouse anti-ox42  (Bio-Rad)

Journal: Frontiers in Molecular Neuroscience

Article Title: C286, an orally available retinoic acid receptor β agonist drug, regulates multiple pathways to achieve spinal cord injury repair

doi: 10.3389/fnmol.2024.1411384

Figure Lengend Snippet: C286 induces functional recovery and tissue sparing in a model of spinal cord contusion. (A) C286 treatment leads to significant improvements in locomotor function in hind limbs in comparison to vehicle-treated rats. Data represent the mean ± SEM of n = 4, * p ≤ 0.01, ** p ≤ 0.005. “____” for the statistical significance of the right hind limbs and “___” for the left. One-way Analysis of Variance (ANOVA), followed by the Fisher test. (B) A schematic representation of the spinal cord area used for analysis of the injury pathology and cavity size. (C) Quantification of cavity area (expressed as a percentage of SC area) at 0 and 1 mm caudal to the epicentre at week 6 post-injury. Data represent the mean ± SEM, ** p ≤ 0.005, *** p ≤ 0.001, and Student's t -test. (D–I) C286 treatment leads to improved injury pathology and neuroprotection following a spinal contusion. Quantification of averaged (E) OX42, (F) NeuN, (H) GFAP, and (I) Iba1 expression shown as mean pixels in arbitrary units (a.u.) measured over a series of sections from 0–1 mm caudal to the epicentre revealed a significant preservation of spinal neurons and decreased reactive glia following C286 treatment compared with vehicle treatment. Data represent the mean FI ± SEM, ** p ≤ 0.005, *** p ≤ 0.001, and Student's t -test.

Article Snippet: They were incubated in the secondary antibody for 1 h at room temperature (RT) in PBS-0.02% Tween and then washed in PBS three times for 5 min. Antibodies used were: mouse monoclonal anti-βIII tubulin (Promega, 1:1,000 for immunohistochemistry), chicken polyclonal anti-GFAP (Abcam, 1:300); rabbit polyclonal anti-RARβ (Santa Cruz Biotechnology, Inc, 1:100 for immunohistochemistry), goat polyclonal anti-CGRP (Abcam, 1:200); mouse monoclonal anti-GAP-43 (Millipore, 1:20); mouse monoclonal anti-NF160 (Sigma Aldrich, 1:200); mouse monoclonal anti-OX42 (Abcam, 1:500); rabbit polyclonal anti-Iba1 (DAKO, 1:1,000); mouse monoclonal anti-CSPG (CS-56) (Sigma, 1:100), goat polyclonal anti-decorin (R&D Systems, 1:20, for immunochemistry and 5μg/ml as a neutralising antibody), rabbit polyclonal anti-decorin (Abcam, 1:50), mouse monoclonal anti-Osteopontin Invitrogen (ThermoFisher, 1:250); mouse monoclonal anti- integrin α9 (Abcam, 1:250); mouse monoclonal anti-tenascin-C (Abcam, 1:300).

Techniques: Functional Assay, Comparison, Expressing, Preserving

Journal: iScience

Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression.

doi: 10.1016/j.isci.2023.106647

Figure Lengend Snippet: Figure 7. Immunohistochemical staining of CD4, CD8, and OX42 in 34-week-old rats in Ctrl, i.p., i.p.m, i.p.b and i.p.mb group with transplantation of hOPCs-1 (A–C) Immunohistochemical staining of CD4, CD8, and OX42 in 34-week-old rats, Scale bar, 10 mm. (D) Histograms of the statistical analysis of the average OD of CD4, CD8, and OX42 in 34-week-old Wistar rats. Independent sample t-test was used for comparison between the experimental group and the control group, *, p＜0.05. Each group has at least three samples. Data are represented as mean G SEM.

Article Snippet: Immunohistochemistry and immunofluorescence staining were performed using the primary antibodies: mouse-anti CD4 (1:100, Cat. #ab33775, Abcam, Cambridge, UK, RRID: AB_726468), mouse-anti CD8 (1:300, Cat. #MCA48G, Bio-Rad, California, USA, RRID: AB_32147), mouse-anti OX42 (1:300, Cat. #MCA275G, Bio-Rad, California, USA, RRID: AB_321301), mouse-anti HNA (1:500, Cat. #MAB1281, Millipore, MA, USA, RRID: AB_94090), mouse-anti Stem121 (1:500, Cat. #Y40410, TaKaRa, Shiga, Japan, RRID: AB_2801314), and rat-anti MBP (1:100, Cat. #MCA409S, Bio-Rad, California, USA, RRID: AB_325004).

Techniques: Immunohistochemical staining, Staining, Transplantation Assay, Comparison, Control

Journal: iScience

Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression.

doi: 10.1016/j.isci.2023.106647

Figure Lengend Snippet: Figure 7. Immunohistochemical staining of CD4, CD8, and OX42 in 34-week-old rats in Ctrl, i.p., i.p.m, i.p.b and i.p.mb group with transplantation of hOPCs-1 (A–C) Immunohistochemical staining of CD4, CD8, and OX42 in 34-week-old rats, Scale bar, 10 mm. (D) Histograms of the statistical analysis of the average OD of CD4, CD8, and OX42 in 34-week-old Wistar rats. Independent sample t-test was used for comparison between the experimental group and the control group, *, p＜0.05. Each group has at least three samples. Data are represented as mean G SEM.

Article Snippet: k Isotype Ctrl (ICFC) Biolegend Cat# 400155; RRID: AB_2832978 mouse-anti CD4 Abcam Cat. #ab33775, RRID: AB_726468 mouse-anti CD8 Bio-Rad Cat. #MCA48G, RRID: AB_32147 mouse-anti OX42 Bio-Rad Cat. #MCA275G, RRID: AB_321301 mouse-anti HNA Millipore Cat. #MAB1281, RRID: AB_94090 mouse-anti Stem121 TaKaRa Cat. #Y40410, RRID: AB_2801314 rat-anti MBP Bio-Rad Cat. #MCA409S, RRID: AB_325004 Alexa 488-conjugated donkey anti-mouse Abcam Cat. #ab150105, RRID: AB_2732856 Alexa 594-conjugated donkey anti-rabbit Abcam Cat. #ab150064, RRID: AB_2734146 Alexa 488-conjugated donkey anti-rabbit Abcam Cat. #ab150153, RRID: AB_2737355 (Continued on next page) iScience 26, 106647, May 19, 2023 19

Techniques: Immunohistochemical staining, Staining, Transplantation Assay, Comparison, Control

Journal: iScience

Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression

doi: 10.1016/j.isci.2023.106647

Figure Lengend Snippet: Immunohistochemical staining of grafts in 10-week-old rats in ctrl, i.p., i.p.m, i.p.b, and i.p.mb group with transplantation of hOPCs-1 (A) Immunohistochemical staining of human nuclear antigen (HNA) in the brain of rats in each group. Scale bar, 20 μm, 10 μm (red box). (B) Immunohistochemical staining of CD4 and OX42 in the corpus callosum in each group. Scale bar, 500 μm. (C) Immunohistochemical staining of CD4 in desensitized muscles (the left forelimbs in i.p.m and i.p.mb groups) and un-desensitized muscles (the right hind limbs in i.p., i.p.m, i.p.b, and i.p.mb groups). Scale bar, 100 μm, 20 μm (red box). i.p., i.p.m, i.p.b, and i.p.mb stand for intraperitoneal, i.p.+muscle, i.p.+brain, and i.p.+muscle+brain, respectively.

Article Snippet: mouse-anti OX42 , Bio-Rad , Cat. #MCA275G, RRID: AB_321301.

Techniques: Immunohistochemical staining, Staining, Transplantation Assay, Muscles

Journal: iScience

Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression

doi: 10.1016/j.isci.2023.106647

Figure Lengend Snippet: Immunohistochemical staining of CD4, CD8, and OX42 in 34-week-old rats in Ctrl, i.p., i.p.m, i.p.b and i.p.mb group with transplantation of hOPCs-1 (A–C) Immunohistochemical staining of CD4, CD8, and OX42 in 34-week-old rats, Scale bar, 10 μm. (D) Histograms of the statistical analysis of the average OD of CD4, CD8, and OX42 in 34-week-old Wistar rats. Independent sample t-test was used for comparison between the experimental group and the control group, ∗, p ＜0.05. Each group has at least three samples. Data are represented as mean ± SEM.

Article Snippet: mouse-anti OX42 , Bio-Rad , Cat. #MCA275G, RRID: AB_321301.

Techniques: Immunohistochemical staining, Staining, Transplantation Assay, Comparison, Control

Journal: iScience

Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression

doi: 10.1016/j.isci.2023.106647

Figure Lengend Snippet:

Article Snippet: mouse-anti OX42 , Bio-Rad , Cat. #MCA275G, RRID: AB_321301.

Techniques: Control, Software, Suspension